Evaluation of High-Throughput Bioanalytical LC-MS/MS Methods: From Development to Validation to Transfer

Abstract

For precise drug, biomarker, and metabolite quantification in biological matrices, it is necessary to

design and validate bioanalytical procedures. Liquid chromatography- tandem mass spectrometry

(LC/MS/MS) is one of the most popular analytical methods used in bioanalysis because of its great

sensitivity, specificity, and adaptability. The development and validation of LC/MS/MS bioanalytical

techniques for the quantitative determination of analytes in various biological fluids, including plasma,

serum, and urine, is the primary emphasis of this work. In order to improve the analysis's performance

in terms of sensitivity, accuracy, and precision, it emphasizes the optimization of essential parameters,

such as procedures for sample preparation, chromatographic conditions, and MS/MS settings. In

addition, the study discusses typical problems that arise during technique development and offers

solutions to these problems, including matrix effects, ion suppression, and interference from

endogenous chemicals.