Bacterial And Serotyping Aspects of Non-0157 Shiga Toxin Producing Escherichia Coli Isolated from Children and Calves in Almuthana Province, Iraq.

    Ahmed Sami Jarad ,Khalil H. Aljeboori

    Abstract

    Bacteriological study was subjected to provide information for non-0157 shiga toxin producing Escherichia coli prevalance  in children and calves using selective media ( chromagar  STEC and chromagar 0157 ) from 127 children with diarrhea and 133 stool samples from calves at AlMuthana province , Characterization depends on culture positive colony on MacConkey agar , Levin's eosin methylene blue agar , staining single colony from growth by gram stain, biochemical tests , indole ,methylred Voges –proskauer , citrate ,oxidase , catalase , urease ,motility , kligler iron and API-20E tests were done to confirm diagnosis of non-0157 STEC  .The reliable isolation as non-0157 serotyping by specific latex agglutination test for target non-0157 STEC  ( big Six ) serogroup (026,045,0103,0111,0121 and 0145) .

    Conclusion The current study revealed that the non-0157 STEC was 20 out of 127 (15.73%) in samples collected from children and 27 out of 133 (20.30%) in calves’ samples.

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