Tricycles constitute a major component of the informal transport system in many Nigerian cities. Their operation is fast becoming a parody to urban living and as such, require urgent regulatory attention. The paper examined the perception of comfort that passengers derive from the use of tricycles for urban mobility in Calabar metropolis, Nigeria. The study modelled different comfort levels based on demographic characteristics of the passengers, as derived from its use. Questionnaire and data from CRDoPT and other published materials were used to make inferences on the study. Analyses was by descriptive and inferential statistics. Ordinal regression analysis using the PLUM method in SPSS analysed data where the null hypothesis that, there is no statistical relationship between passenger demographic parameters and the feeling of comfort from the use of the tricycle in Calabar, was rejected. The feeling of comfort of the tricycle mode users is observed to be dependent (p < .05) on the education (p = .000); monthly income (p = .001); occupation (p = .046); and marital status (p = .003) on the respondents. The study recommended among other things, regulatory policies that are expected to enhance an effective modal regulation that promotes a safe and comfortable use of the mode. Registration and creation of an accurate database of the tricycle operators and operations in order to formalize tricycle operation can enhance the confidence of use and boost comfort levels of passengers.